县级实验室应用结核分枝杆菌手工液体培养技术检测结果分析

doi:10.19983/j.issn.2096-8493.20210136

结核与肺部疾病杂志 ›› 2022, Vol. 3 ›› Issue (2): 173-176.doi: 10.19983/j.issn.2096-8493.20210136

• 短篇论著 • 上一篇

县级实验室应用结核分枝杆菌手工液体培养技术检测结果分析

邱勇¹(), 李蒙², 屈榕¹, 田发君¹

¹四川省广安市武胜县疾病预防控制中心结防科,广安 638401
²复旦大学基础医学院教育部、卫健委、医科院分子病毒学重点实验室,上海 200032

收稿日期:2021-10-26 出版日期:2022-06-30 发布日期:2022-04-18
通信作者: 邱勇 E-mail:48584409@qq.com

Analysis of manual liquid culture method in county-level laboratory to detect Mycobacterium tuberculosis

QIU Yong¹(), LI Meng², QU Rong¹, TIAN Fa-jun¹

¹Department of Tuberculosis Control, Center for Disease Control and Prevention in Wusheng County, Sichuan Province, Guang’an 638401, China
²Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Science, Shanghai Medical College, Fudan University, Shanghai 200032, China

Received:2021-10-26 Online:2022-06-30 Published:2022-04-18
Contact: QIU Yong E-mail:48584409@qq.com

摘要/Abstract

摘要：

收集2018年7月1日至2020年12月31日在四川省武胜县疾病预防控制中心结核病防治门诊就诊的1370例疑似肺结核患者的痰标本,分别使用手工液体培养法与中和离心固体培养法进行培养。手工液体培养法的阳性率为25.8%(353/1370),显著高于中和离心固体培养法的20.0%(274/1370)(χ²=12.907,P<0.001)。手工液体培养法的报告阳性中位时间为2(2,3)周,显著短于中和离心固体培养法的3(2,4)周(Z=-9.491,P<0.001)。手工液体培养法显著提高了报告阳性率,显著缩短报告阳性时间,而且试剂成本与固体培养成本相当,适合在县级实验室应用。

关键词: 实验室技术和方法, 分枝杆菌, 结核, 培养技术

Abstract:

Sputum specimens were collected from 1370 presumptive tuberculosis patients attending to the tuberculosis clinic of Center for Disease Control and Prevention in Wusheng County of Sichuan Province from July 1, 2018 to December 31, 2020. The sputum was cultured using manual liquid culture method and neutralization centrifugal solid culture method at the same time. The positive rate of the manual liquid culture method was 25.8% (353/1370), significantly higher than that of the neutralization centrifugal solid culture method (20.0%, 274/1370) (χ²=12.907, P<0.001). The median time to detection was 2 (2, 3) weeks for the manual liquid culture method, which was significantly shorter than that for the solid culture method (3 (2, 4) weeks, Z=-9.491, P<0.001). The manual liquid culture method significantly improved the positive rate, significantly shortened the time to detection, and was comparable in cost to solid culture, making it suitable for application in county-level laboratory.

Key words: Laboratory techniques and procedures, Mycobacterium tuberculosis, Culture techniques

中图分类号:

R521

邱勇, 李蒙, 屈榕, 田发君. 县级实验室应用结核分枝杆菌手工液体培养技术检测结果分析[J]. 结核与肺部疾病杂志, 2022, 3(2): 173-176. doi: 10.19983/j.issn.2096-8493.20210136

QIU Yong, LI Meng, QU Rong, TIAN Fa-jun. Analysis of manual liquid culture method in county-level laboratory to detect Mycobacterium tuberculosis[J]. Journal of Tuberculosis and Lung Disease, 2022, 3(2): 173-176. doi: 10.19983/j.issn.2096-8493.20210136

图/表 2

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手工MFLM 培养	中和离心固体培养			合计
手工MFLM 培养	阳性	阴性	污染	合计
阳性	262	62	29	353
阴性	12	802	61	875
污染	0	88	54	142
合计	274	952	144	1370

报告阳性时间	手工MFLM液体培养		报告阳性时间	中和离心固体培养
报告阳性时间	报告阳性例数	报告阳性率(%)	报告阳性时间	报告阳性例数	报告阳性率(%)
0.5周	34	9.6	1周	7	2.6
1周	50	23.8	2周	68	27.4
1.5周	0	23.8	3周	106	66.1
2周	137	62.6	4周	55	86.1
3周	79	85.0	5周	15	91.6
4周	35	94.9	6周	10	95.3
5周	8	97.2	7周	8	98.2
6周	10	100.0	8周	5	100.0
合计	353			274

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县级实验室应用结核分枝杆菌手工液体培养技术检测结果分析

Analysis of manual liquid culture method in county-level laboratory to detect Mycobacterium tuberculosis

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