两种实验室技术对肺结核患者手术后不同组织标本的检测结果分析

doi:10.3969/j.issn.2095-3755.2020.01.017

结核病与肺部健康杂志 ›› 2020, Vol. 9 ›› Issue (1): 79-81.doi: 10.3969/j.issn.2095-3755.2020.01.017

两种实验室技术对肺结核患者手术后不同组织标本的检测结果分析

宫亚娅, 鲁怀伟, 王琦, 操乐杰()

230001 合肥,安徽医科大学省立临床学院 [宫亚娅(研究生)];安徽医科大学附属省立医院呼吸科(宫亚娅、操乐杰),检验科(鲁怀伟),病理科(王琦)

收稿日期:2019-12-20 出版日期:2020-03-30 发布日期:2020-04-17
通信作者: 操乐杰 E-mail:sycaolejie@163.com

Analysis of specimens examination results of patients with tuberculosis after surgery by using two laboratory techniques

GONG Ya-ya, LU Huai-wei, WANG Qi, CAO Le-jie()

^*Department of Respiratory Disease, Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, China (^*Work unit)

Received:2019-12-20 Online:2020-03-30 Published:2020-04-17
Contact: CAO Le-jie E-mail:sycaolejie@163.com

摘要/Abstract

摘要：

收集2017年6月至2019年1月就诊于安徽医科大学附属省立医院胸外科,获得肺部组织标本的60例疑诊肺结核患者作为研究对象。对比患者肺部组织病理标本GeneXpert MTB/RIF和抗酸染色的检测结果,评估GeneXpert MTB/RIF和抗酸染色对肺结核患者手术组织标本的检测价值。60例患者中,GeneXpert MTB/RIF检测和抗酸染色的阳性检出率分别为46.7%(28/60)和48.3%(29/60)。5例标本抗酸染色呈阳性,GeneXpert MTB/RIF检测呈阴性,通过分枝杆菌菌种鉴定提示均为结核分枝杆菌。两种方法联合检测的阳性检出率为55.0%(33/60),达到“十三五”全国结核病防治规划要求的病原学检出率提高至50%的目标。研究结论认为,GeneXpert MTB/RIF与抗酸染色方法联合检测肺结核患者肺组织标本,阳性率较高,具有一定的临床应用价值。

关键词: 结核,肺, 病理学, 核酸扩增技术, 诊断技术和方法

Abstract:

A total of 60 patients, who had been suspected diagnosed as tuberculosis (TB) and received surgery at department of thoracic surgery of Anhui Provincial Hospital from June 2017 to January 2019, were enrolled in this study. The lung tissue specimens were obtained from those enrolled patients. GeneXpert MTB/RIF and acid-fast staining tests were performed on all patients’ pathological specimens to evaluate the value of GeneXpert MTB/RIF and acid-fast staining on detection of pulmonary TB with their surgical tissue specimens. In 60 patients, the positive rate of GeneXpert MTB/RIF and acid-fast staining was 46.7% (28/60) and 48.3% (29/60) respectively. Five specimens with acid-fast staining positive but GeneXpert MTB/RIF negative were confirmed to be Mycobacterium tuberculosis (MTB) by mycobacteria identification. The positive rate of both GeneXpert MTB/RIF and acid-fast staining was 55.0% (33/60), which met the target of 50% pathogenic detection rate at least required in the 13th Five-year National TB Control Program. In conclusion, the TB detection rate is high by jointly using GeneXpert MTB/RIF and acid-fast staining and it’s valuable in clinical diagnosis of TB.

Key words: Tuberculosis, pulmonary, Pathology, Nucleic acid amplification techniques, Diagnostic techniques and procedures

宫亚娅, 鲁怀伟, 王琦, 操乐杰. 两种实验室技术对肺结核患者手术后不同组织标本的检测结果分析[J]. 结核病与肺部健康杂志, 2020, 9(1): 79-81. doi: 10.3969/j.issn.2095-3755.2020.01.017

GONG Ya-ya, LU Huai-wei, WANG Qi, CAO Le-jie. Analysis of specimens examination results of patients with tuberculosis after surgery by using two laboratory techniques[J]. Journal of Tuberculosis and Lung Health, 2020, 9(1): 79-81. doi: 10.3969/j.issn.2095-3755.2020.01.017

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两种实验室技术对肺结核患者手术后不同组织标本的检测结果分析

Analysis of specimens examination results of patients with tuberculosis after surgery by using two laboratory techniques

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