同位素标记相对和绝对定量结合二维液相色谱-串联质谱技术在结核病诊断中的应用

doi:10.3969/j.issn.2095-3755.2018.03.015

结核病与肺部健康杂志 ›› 2018, Vol. 7 ›› Issue (3): 217-220.doi: 10.3969/j.issn.2095-3755.2018.03.015

同位素标记相对和绝对定量结合二维液相色谱-串联质谱技术在结核病诊断中的应用

哈斯来提阿依·买买提,胡昕,齐曼古力·吾守尔()

830054 乌鲁木齐,新疆医科大学第一附属医院呼吸科

收稿日期:2018-04-13 出版日期:2018-09-30 发布日期:2018-10-16
通信作者: 齐曼古力·吾守尔 E-mail:qimenw@hotmail.com
基金资助:
新疆维吾尔自治区重大科技专项(2017A03006-2);新疆地区肺结核患者血浆蛋白质组学的相关研究(SKL-HIDCA-2017-Y12)

Research progress of isobaric tags for relative and absolute quantitation and two-dimensional liquid chromatography-tandem mass spectrometry in the diagnosis of tuberculosis

Xin HU,Wushouer Qimanguli·()

Department of Respiratory,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China

Received:2018-04-13 Online:2018-09-30 Published:2018-10-16
Contact: Wushouer Qimanguli· E-mail:qimenw@hotmail.com

摘要/Abstract

摘要：

近几年,随着生物技术的发展,结核病诊断的研究重点逐渐转向能及时反映机体状态的血清蛋白质组学研究,并通过蛋白质组学技术发现结核病患者血清中的差异蛋白,为疾病早期诊断及鉴别诊断提供重要的理论依据。同位素标记相对和绝对定量(iTRAQ)结合二维液相色谱-串联质谱(2DLC-MS/MS)技术因分离能力强、分析范围广、敏感度高、特异度高、检测限低等诸多优点,受研究人员关注并广泛使用。作者对基于iTRAQ-2DLC-MS/MS技术的结核病患者血清蛋白质组学的研究予以综述。

关键词: 结核, 蛋白质组学, 同位素标记, 色谱法, 液相, 串联质谱法, 实验室技术和方法, 综述文献(主题)

Abstract:

In recent years, with the development of biotechnology, the study focus on tuberculosis diagnosis has shifted to serum proteomics research that can reflect the state of organism in time and identify differential proteins in the serum of tuberculosis patients, providing an important theoretical basis for early diagnosis and differential diagnosis of disease. Isobaric tags for relative and absolute quantitation and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) technology is concerned and widely used by researchers because of its strong separation ability, wide analysis range, high sensitivity, high specificity, and low detection limit. This article reviews the researches on serum proteomics of tuberculosis patients based on iTRAQ-2DLC-MS/MS technology.

Key words: Tuberculosis, Proteomics, Isotope labeling, Chromatography, liquid, Tandem mass spectrometry, Laboratory techniques and procedures, Review literature as topic

哈斯来提阿依·买买提,胡昕,齐曼古力·吾守尔. 同位素标记相对和绝对定量结合二维液相色谱-串联质谱技术在结核病诊断中的应用[J]. 结核病与肺部健康杂志, 2018, 7(3): 217-220. doi: 10.3969/j.issn.2095-3755.2018.03.015

Xin HU,Wushouer Qimanguli·. Research progress of isobaric tags for relative and absolute quantitation and two-dimensional liquid chromatography-tandem mass spectrometry in the diagnosis of tuberculosis[J]. Journal of Tuberculosis and Lung Health, 2018, 7(3): 217-220. doi: 10.3969/j.issn.2095-3755.2018.03.015

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同位素标记相对和绝对定量结合二维液相色谱-串联质谱技术在结核病诊断中的应用

Research progress of isobaric tags for relative and absolute quantitation and two-dimensional liquid chromatography-tandem mass spectrometry in the diagnosis of tuberculosis

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