儿童结核病分子生物学诊断方法研究进展

doi:10.3969/j.issn.2096-8493.2021.01.015

结核与肺部疾病杂志 ›› 2021, Vol. 2 ›› Issue (1): 69-72.doi: 10.3969/j.issn.2096-8493.2021.01.015

儿童结核病分子生物学诊断方法研究进展

焦伟伟()

100045 首都医科大学附属北京儿童医院北京市儿科研究所儿科学国家重点学科儿科重大疾病研究教育部重点实验室国家呼吸系统疾病临床医学研究中心儿童呼吸道感染性疾病研究北京市重点实验室

收稿日期:2021-02-22 出版日期:2021-03-30 发布日期:2021-03-24
通信作者: 焦伟伟 E-mail:Jiaowei310@163.com
基金资助:
首都卫生发展科研专项(首发2020-2-1142);北京市优秀人才培养资助青年拔尖个人项目(2016000021223ZK38)

Advances in molecular diagnostic techniques of tuberculosis in children

JIAO Wei-wei()

MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University,Beijing 100045, China

Received:2021-02-22 Online:2021-03-30 Published:2021-03-24
Contact: JIAO Wei-wei E-mail:Jiaowei310@163.com

摘要/Abstract

摘要：

结核病是严重危害儿童健康的重要疾病,但诊断工作面临诸多困难。随着分子生物学检测技术的发展,越来越多的新的结核病诊断方法涌现出来,包括GeneXpert MTB/RIF、超敏结核分枝杆菌和利福平耐药基因检测方法(GeneXpert MTB/RIF Ultra)、宏基因组测序(mNGS),以及基于恒温扩增技术和基于宿主的结核病分子诊断方法,笔者针对这些方法在儿童结核病中的应用作一综述。

关键词: 结核, 儿童, 分子诊断技术, 综述文献(主题)

Abstract:

Tuberculosis is a major threat to children’s health. However, there are many difficulties in the diagnosis of tuberculosis in children. With the development of molecular biology technique, more and more new diagnostic methods for tuberculosis have emerged, including GeneXpert MTB/RIF, GeneXpert MTB/RIF ultra, metagenomic next-generation sequencing (mNGS) and methods based on isothermal amplification, as well as host based molecular diagnostic methods. This paper reviews the application of these methods in children.

Key words: Tuberculosis, Children, Molecular diagnostic techniques, Review literature as topic

焦伟伟. 儿童结核病分子生物学诊断方法研究进展[J]. 结核与肺部疾病杂志, 2021, 2(1): 69-72. doi: 10.3969/j.issn.2096-8493.2021.01.015

JIAO Wei-wei. Advances in molecular diagnostic techniques of tuberculosis in children[J]. Journal of Tuberculosis and Lung Disease, 2021, 2(1): 69-72. doi: 10.3969/j.issn.2096-8493.2021.01.015

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儿童结核病分子生物学诊断方法研究进展

Advances in molecular diagnostic techniques of tuberculosis in children

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