Journal of Tuberculosis and Lung Disease ›› 2021, Vol. 2 ›› Issue (1): 8-12.doi: 10.3969/j.issn.2096-8493.2021.01.003
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Received:
2021-02-10
Online:
2021-03-30
Published:
2021-03-24
Contact:
HAN Li-jun
E-mail:2301617255@qq.com
HAN Li-jun, ZHAO Xue-yao. Molecular detection technologies for diagnosing tuberculous meningitis using cerebrospinal fluid[J]. Journal of Tuberculosis and Lung Disease , 2021, 2(1): 8-12. doi: 10.3969/j.issn.2096-8493.2021.01.003
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URL: http://www.jtbld.cn/EN/10.3969/j.issn.2096-8493.2021.01.003
比较项目 | GeneXpert | GeneXpert MTB/RIF Ultra | LAMP | 熔解曲线技术 | 线性探针技术 |
---|---|---|---|---|---|
检测原理 | 实时荧光定量PCR | 实时荧光定量PCR | 等温扩增技术 | 探针-熔解曲线技术 | 探针-反向杂交技术 |
检测位点 | rpoB[ | IS6110、IS1081、 ropB[ | IS6110、gyrB[ | rpoB、katG、inhA、oxyR、abpC、gyrA[ | rpoB、katG、inhA[ |
报告时间 | 2h[ | 2h[ | 1h[ | 72h[ | 6h[ |
检测内容 | 结核分枝杆菌复合群,利福平耐药性[ | 结核分枝杆菌复合群,利福平耐药性[ | 结核分枝杆菌复合群[ | 结核分枝杆菌复合群,异烟肼、利福平、乙胺丁醇、氟喹诺酮类药品耐药性[ | 结核分枝杆菌复合群,异烟肼、利福平的耐药性(2代还可检测氟喹诺酮类及二线注射类药品的耐药性)[ |
敏感度 | 23%~52%[ | 89.40%[ | 43.2%~82%[ | 12.9%[ | 96.15%[ |
特点 | 需专门的仪器,手动操作简便,可检测利福平耐药性,结果可靠[ | 需专门的仪器,手动操作简便,可检测利福平耐药性,结果可靠,较GeneXpert检测阈值低[ | 操作简便,对实验室及检测人员要求低[ | 可检测多种药物耐药性,但脑脊液相关检测数据较少,难以评价其实用性 | 可检测多种药物耐药性,操作复杂,对实验室及检测人员要求高[ |
WHO是 否推荐 | 是[ | 是[ | 推荐应用于痰液检测,肺外结核的检测有待进一步研究[ | 否 | 仅推荐应用于痰液检测[ |
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