结核病比较免疫学时代的机遇和挑战

doi:10.3969/j.issn.2096-8493.2020.03.002

摘要/Abstract

摘要：

结核病领域一系列关键技术的突破,特别是结核分枝杆菌基因敲除突变体文库和系统的结核病动物模型,为结核病比较免疫学的形成和发展提供了可能。而结核病比较免疫学数据的积累,将为结核病免疫机理研究和结核病新型疫苗的研发创造新的机遇。作者从技术层面,对结核病比较免疫学的数据框架方式和发展方向进行了系统的描述和讨论。

关键词: 结核, 免疫学技术, 模型,动物, 基因敲除技术, 疫苗, 比较免疫学

Abstract:

With the breakthrough of key technologies, the achievements based on the gene knockout MTB mutant library and animal models of tuberculosis, enable the development of systematic immunology of tuberculosis (SIT). In this review, we described the data framework of SIT, and we also discussed how the SIT database will benefit the prosperity of novel vaccine and drug development against the tuberculosis disease.

Key words: Tuberculosis, Immunologic techniques, Animal models, Gene knockout techniques, Vaccines, Systematic immunology

朱国峰, 刘晓清. 结核病比较免疫学时代的机遇和挑战[J]. 结核与肺部疾病杂志, 2020, 1(3): 195-212. doi: 10.3969/j.issn.2096-8493.2020.03.002

ZHU Guo-feng, LIU Xiao-qing. The new era of systematic immunology of tuberculosis[J]. Journal of Tuberculosis and Lung Disease, 2020, 1(3): 195-212. doi: 10.3969/j.issn.2096-8493.2020.03.002

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领域	数据类型		数据构成	结核病比较免疫学数据贡献	价值意义
临床	结核病临床分类患者免疫数据		临床结核病体液免疫学(B细胞) 临床结核病细胞免疫学(T细胞)	临床应用	理论方面: 形成“结核病比较免疫学”
核心实验技术	结核病动物模型	猴子	猴子结核病系统免疫学		应用方面:
		豚鼠	豚鼠结核病系统免疫学	结核病比较免疫学	(1)诊断: 高敏感度免疫诊断技术
		小鼠	小鼠结核病系统免疫学	核心数据框架	(2)疫苗: 保护性免疫反应数据库
	MTB基因敲除	MTB	MTB基因敲除突变体(约4000个+野生型H37Rv)		(3)药物: 宿主免疫调控及新药物