非痰液样本结核分枝杆菌游离DNA检测在结核病诊断中的研究进展 基金项目:陕西省卫生健康科研项目(2021D027)

doi:10.3969/j.issn.2096-8493.20210069

摘要/Abstract

摘要：

结核病是结核分枝杆菌引起的慢性传染病,以肺结核最为常见。实验室检查主要以呼吸道样本(痰液、灌洗液)为检测对象,但对儿童结核病、肺外结核和并发HIV/AIDS患者的检测效果并不理想,故为提高这些人群的检测阳性率,以非痰液样本为基础的新的诊断方法也在不断研究中。游离DNA(cfDNA)是从原始细胞释放到各种体液中的DNA片段,对多种疾病的诊断具有广阔的应用前景。笔者针对血液或尿液等样本中结核分枝杆菌cfDNA的检测潜力,就其应用现状、提取前处理影响因素、提取方法和检测方法等方面的最新研究并结合经验进行论述。

关键词: 分枝杆菌,结核, DNA, 病原学诊断, 尿液, 血液, 综述文献(主题)

Abstract:

Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis, pulmonary tuberculosis is the most common. Laboratory test mainly respiratory specimens (sputum, lavage) as test objects, however, the test effective is unfavorable for children, extrapulmonary tuberculosis, and TB/HIV or AIDS patients. Thus, new diagnosis method based on non-sputum samples in continuous research in order to positive test rate of above population. Free DNA (cfDNA) is a DNA fragment released from primitive cells into various body fluids, which has a broad application prospect in the diagnosis of various diseases. In the article, application, factors affecting extraction pretreatment, extraction and detection methods of Mycobacterium tuberculosis cfDNA detection in blood or urine specimen will be discussed by authors, in combination with literature and clinical experience.

Key words: Mycobacterium tuberculosis, DNA, Etiological diagnosis, Brine, Blood, Review literature as topic

李静, 张燕, 仵倩红. 非痰液样本结核分枝杆菌游离DNA检测在结核病诊断中的研究进展基金项目:陕西省卫生健康科研项目(2021D027)[J]. 结核与肺部疾病杂志, 2021, 2(3): 277-282. doi: 10.3969/j.issn.2096-8493.20210069

LI Jing, ZHANG Yan, WU Qian-hong. Research progress on free DNA detection of Mycobacterium tuberculosis of non-sputum samples in tuberculosis diagnosis[J]. Journal of Tuberculosis and Lung Disease, 2021, 2(3): 277-282. doi: 10.3969/j.issn.2096-8493.20210069

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