2010年驻京部队入伍新兵结核潜伏感染情况的调查

结核病与肺部健康杂志 ›› 2012, Vol. 1 ›› Issue (2): 105-107.

2010年驻京部队入伍新兵结核潜伏感染情况的调查

梁艳,吴雪琼,王兰,王志耘,张翠英,阳幼荣,张俊仙

100091 北京,解放军第三〇九医院全军结核病研究所军队结核病防治重点实验室

收稿日期:2012-06-23 出版日期:2012-10-20 发布日期:2012-10-20
通信作者: 吴雪琼, Email:wu-xueqiong@263.net
基金资助:
“十一五”国家重大科技专项(2008ZX-10003-001)

An investigation on the latent tuberculosis infection in the new recruits from Beijing army with enzyme-linked immuno-spot assay in 2010

LIANG Yan,WU Xue-qiong,WANG Lan,WANG Zhi-yun,ZHANG Cui-ying,YANG You-rong,ZHANG Jun-xian

Army Tuberculosis Key Laboratory, Tuberculosis Research Institute,the 309th Hospital of Chinese PLA, Beijing 100091, China

Received:2012-06-23 Online:2012-10-20 Published:2012-10-20
Contact: WU Xue-qiong,Email: wu-xueqiong@263.net

摘要/Abstract

摘要： 目的应用酶联免疫斑点试验(enzyme linked-immunospot assay, ELISPOT)检测入伍新兵结核潜伏感染情况,评价ELISPOT在检测结核潜伏感染中的价值。方法以结核菌素纯蛋白衍生物 (PPD) 皮肤试验为对照,应用ELISPOT试剂盒检测128例2010年驻京部队入伍新兵外周血中分泌结核分枝杆菌抗原特异性γ干扰素(IFN-γ)的T淋巴细胞数,对于PPD和ELISPOT均为阴性的45例入伍新兵接种卡介苗。结果 128例入伍新兵中,PPD皮肤试验和ELISPOT阳性率分别为50.00%(64/128)和28.13%(36/128)。64例PPD皮肤试验阴性和64例PPD皮肤试验阳性者中,分别有19例 (29.69%)和17例(26.56%)ELISPOT阳性,两者的一致率为48.44%(62/128),两者的检测结果差异具有统计学意义(χ²=9.766,P=0.0018)。在有卡介苗接种者中,PPD皮肤试验阳性率为57.44%(58/101),ELISPOT阳性率为26.73%(27/101)[斑点形成细胞数(SFC):23.52±11.01];在无卡介苗接种者中,PPD皮肤试验阳性率为22.22%(6/27),ELISPOT阳性率为33.33%(9/27)(SFC:32.78±24.06)。结论 ELISPOT具有较好的特异度和敏感度,能够更有效地筛查结核分枝杆菌感染。

Abstract: Objective To study the latent tuberculosis infection (LTBI) in the new recruits from Beijing army with enzyme-linked immunospot assay (ELISPOT), and to evaluate the value of ELISPOT assay.Methods A total of 128 new recruits from Beijing army in 2010 were examined by purified protein derivative (PPD) skin test, and the number of T cells secreting IFN-γ in their peripheral blood in recombinant CFP-10/ESAT-6 fusion protein (rCFP-10/ESAT-6) were detected by ELISPOT assay. 45 new recruits who were negative by ELISPOT and PPD skin test were vaccinated with BCG vaccine.Results Of 128 new recruits, the positive rates of PPD skin test and ELISPOT assay were 50.00% (64/128) and 28.13% (36/128), respectively. Of 64 PPD-negative and 64 PPD-positive new recruits, 19 (29.69%) and 17 (26.56%) were ELISPOT positive, respectively. Overall agreement between these two tests was 48.44% (62/128), but their difference was statistically remarkable (χ²=9.776,P=0.0018). Of the new recruits found the vaccination scars on their arms, 57.44% (58/101) was PPD-positive, and 26.73% (27/101) was ELISPOT-positive [spot forming cell (SFC):23.52±11.01].Of the new recruits without the vaccination scars on their arms, 22.22% (6/27) was PPD-positive, and 33.33% (9/27) was ELISPOT-positive (SFC:32.78±24.06).Conclusion ELISPOT technique has higher specificity and sensitivity for screening LTBI.

梁艳,吴雪琼,王兰,王志耘,张翠英,阳幼荣,张俊仙. 2010年驻京部队入伍新兵结核潜伏感染情况的调查[J]. 结核病与肺部健康杂志, 2012, 1(2): 105-107.

LIANG Yan,WU Xue-qiong,WANG Lan,WANG Zhi-yun,ZHANG Cui-ying,YANG You-rong,ZHANG Jun-xian. An investigation on the latent tuberculosis infection in the new recruits from Beijing army with enzyme-linked immuno-spot assay in 2010[J]. Journal of Tuberculosis and Lung Health, 2012, 1(2): 105-107.