Abstract: Objective To compare the diagnostic value of detecting Mycobacterium tuberculosis(Mtb)with fluorogenic quantitative PCR(polymerase chain reaction)and double antigen sandwich colloidal gold immunochromatography.Methods Seventy cases with culture-positive pulmonary tuberculosis were selected to compare the sensitivities,specificities and accuracies of fluorogenic quantitative PCR and double antigen sandwich colloidal gold immunochromatography respectively. SPSS 12.0 software was applied for data cleaning and analysis,and P<0.05 was set as the criteria of statistical significance.Results The bacterial culture results were regarded as the gold stan-dard, the results of fluorogenic quantitative PCR(the former)and double antigen sandwich colloidal gold immunochromatography(the latter)were compared. The false-positive rate of the former was significantly lower than the latter [the former:1.7%(1/59),the latter:8.8%(6/68). χ²=5.46,P<0.05]. It meant the specificity of the former was relative higher. The false-negative rate of the former was significantly higher than the latter [the former:15.7%(11/70),the latter:2.9%(2/70). χ²=9.71,P<0.05]. The sensitivity of the latter was higher. There was no statistical significance of the difference between the accuracies of the two methods [the former:82.9%(58/70),the latter:88.6%(62/70). χ²=1.33,P>0.05].Conclusion Both methods are effective and rapid clinical methods to detect Mtb. The fluorogenic quantitative PCR has a more remarkable effect in primary screening with its higher sensitivity, and the double antigen sandwich colloidal gold immunochromatography technique can be a reference for diffe-rential diagnosis with its higher specificity. Both of them have advantages and disadvantages,and they should be selected according to the clinical practice.