Abstract: Objective To explore multidrug resistance tuberculosis and Mycobacterium Strain distribution in Bin Zhou region.Methods Two thousand and eighty-three culture positive cases from outpatient and hospitalized tuberculosis patients between January 2013 and March 2015 were collected, among them, 1765 cases of the initial treatment, 318 cases of the re-treatment.Patients were divided into 5 age groups, 216 cases in 18－ age group,309 cases in 31－ age group, 593 cases in 41－ age group, 434 cases in 51- age group, 531 cases in more than 60 age group. Two thousand and eighty-three cases were conducted identification of multidrug detection and Mycobacterium species identification by Gene chip.Results Two thousand and sixteen strains were Mycobacterium tuberculosis in 2083 Mycobacterium by Gene chip, accounting for 96.8%. The total resistance rate was 3.6% (73/2016). The rate of multi-drug resistance in the initial treatment patients was 1.6% (27/1698) and that in re-treatment patients was 14.5% (46/318); there were 13 multidrug resistance cases in 18－ age group,8 cases in 31－ age group, 12 cases in 41－ age group, 29 cases in 51－ age group, 11 cases in more than 60 age group. a total of 40 cases were more than 51 years old, accounting for 54.8% (40/73). Sixty-seven cases non-Mycobacterium tuberculosis were detected in 2083 Mycobacterium, accounting for 3.2%, among them, 29 strains of Intracellular Mycobacterium, accounting for 43.3%.Conclusion The multidrug resistant rate of tuberculosis in Bin Zhou region was lower than the national average, and the non mycobacterium tuberculosis disease incidence rate was low, mainly intracellular mycobacteria infection.