Effect of different expressions of ERCC1 and LRP performed on the effectiveness of cisplatin in the treatment of malignant pleural effusion

doi:10.3969/j.issn.2095-3755.2019.03.006

Abstract

Abstract:

Objective To investigate the effect of different expressions of excision repair cross complementing 1 (ERCC1))and lung resistance protein (LRP) genes in the treatment of malignant pleural effusion with cisplatin.Methods One hundred and thirty-seven cases with malignant pleural effusion treated in the Oncology Department of Harbin Chest Hospital from January 2016 to June 2019 who refused systemic chemotherapy and received cisplatin injection only into the chest cavity were selected. Within them, 124 cases with tumor cells found in buried section of their pleural effusion samples were enrolled in this study. We used immunohistochemical staining of Streptomyces antibiotin protein-peroxidase ligation(SP method)(abbreviated as “immunohistochemical”) method to detect ERCC1 and LRP genes, then divided patients into 4 groups: 28 cases in group A (low expression of ERCC1 and LRP),38 cases in group B(high expression of ERCC1 and LRP), 35 cases in group C (low expression of ERCC1 and high expression of LRP) and 23 cases in group D(high expression of ERCC1 and low expression of LRP). Defining complete and partial remission as effective treatment, we compared therapeutic effectiveness among groups with different gene combinations. SPSS 18.0 statistical software was used for analysis. Comparison of metrological data between groups was conducted using single factor variance analysis;Comparison of counting data between groups was done with χ ² test. Difference less than 0.05 were considered as statistically significant. Results The topical medication effectiveness of cisplatin in group A, group B, group C, and group D were 85.71% (24/28),26.32% (10/38),51.44% (18/35) and 56.52% (13/23) respectively. Difference between those four groups was statistically significant (χ ²=22.995,P=0.000).The curative rate of group A was higher than that of groups B, C and D (χ ²=22.772,8.229,5.403;P=0.000,0.007,0.029). Curative rate of group B was significantly lower than groups C and D (χ ²=4.860,5.566;P=0.033,0.029).Group C and group D were of equivalent effectiveness (χ ²=0.145,P=0.197). Conclusion The curative effectiveness of 4 groups showed that group A got the best effectiveness, group C and group D had the second best curative effect, and group B had the lowest curative effect. For patients with highly expressed ERCC1 and LRP, we should consider resistance to cisplatin and recommend replacing cisplatin with other drugs.

Key words: Pleural effusion, malignant, Gene expression, Cisplatin, Comparative effectiveness research, Resistance genes

CHANG Li,LIN Hong,SUN Su-qin,PANG You-quan,BIAN Nan-nan,ZHEN Chun-ying,GUAN Xue-qing,YANG Hao,LIU Xue-mei. Effect of different expressions of ERCC1 and LRP performed on the effectiveness of cisplatin in the treatment of malignant pleural effusion[J]. Journal of Tuberculosis and Lung Health , 2019, 8(3): 178-182. doi: 10.3969/j.issn.2095-3755.2019.03.006

Figures/Tables 2

一般情况	A组(28例)	B组(38例)	C组(35例)	D组(23例)	合计(124例)	检验值	P值
年龄(岁, $x ˉ$ ±s)	61.71±10.93	63.16±11.69	62.57±12.25	61.3±10.63	62.32±11.38	F=0.159	0.924
性别[例(构成比,%)]						χ²=2.193	0.533
男	12(42.86)	18(47.37)	20(57.14)	9(39.13)	59(47.58)
女	16(57.14)	20(52.63)	15(42.86)	14(60.87)	65(52.42)
原发病[例(构成比,%)]						χ²=0.681	0.995
肺癌	22(78.57)	28(73.68)	27(77.14)	18(78.26)	95(76.61)
胸膜间皮瘤	4(14.29)	6(15.79)	4(11.43)	3(13.04)	17(13.71)
其他	2(7.14)	4(10.53)	4(11.43)	2(8.70)	12(9.68)

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