Analysis of manual liquid culture method in county-level laboratory to detect Mycobacterium tuberculosis

doi:10.19983/j.issn.2096-8493.20210136

Abstract

Abstract:

Sputum specimens were collected from 1370 presumptive tuberculosis patients attending to the tuberculosis clinic of Center for Disease Control and Prevention in Wusheng County of Sichuan Province from July 1, 2018 to December 31, 2020. The sputum was cultured using manual liquid culture method and neutralization centrifugal solid culture method at the same time. The positive rate of the manual liquid culture method was 25.8% (353/1370), significantly higher than that of the neutralization centrifugal solid culture method (20.0%, 274/1370) (χ²=12.907, P<0.001). The median time to detection was 2 (2, 3) weeks for the manual liquid culture method, which was significantly shorter than that for the solid culture method (3 (2, 4) weeks, Z=-9.491, P<0.001). The manual liquid culture method significantly improved the positive rate, significantly shortened the time to detection, and was comparable in cost to solid culture, making it suitable for application in county-level laboratory.

Key words: Laboratory techniques and procedures, Mycobacterium tuberculosis, Culture techniques

CLC Number:

R521

QIU Yong, LI Meng, QU Rong, TIAN Fa-jun. Analysis of manual liquid culture method in county-level laboratory to detect Mycobacterium tuberculosis[J]. Journal of Tuberculosis and Lung Disease , 2022, 3(2): 173-176. doi: 10.19983/j.issn.2096-8493.20210136

Figures/Tables 2

References 14

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手工MFLM 培养	中和离心固体培养			合计
手工MFLM 培养	阳性	阴性	污染	合计
阳性	262	62	29	353
阴性	12	802	61	875
污染	0	88	54	142
合计	274	952	144	1370

报告阳性时间	手工MFLM液体培养		报告阳性时间	中和离心固体培养
报告阳性时间	报告阳性例数	报告阳性率(%)	报告阳性时间	报告阳性例数	报告阳性率(%)
0.5周	34	9.6	1周	7	2.6
1周	50	23.8	2周	68	27.4
1.5周	0	23.8	3周	106	66.1
2周	137	62.6	4周	55	86.1
3周	79	85.0	5周	15	91.6
4周	35	94.9	6周	10	95.3
5周	8	97.2	7周	8	98.2
6周	10	100.0	8周	5	100.0
合计	353			274

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