Research progress of isobaric tags for relative and absolute quantitation and two-dimensional liquid chromatography-tandem mass spectrometry in the diagnosis of tuberculosis

doi:10.3969/j.issn.2095-3755.2018.03.015

Abstract

Abstract:

In recent years, with the development of biotechnology, the study focus on tuberculosis diagnosis has shifted to serum proteomics research that can reflect the state of organism in time and identify differential proteins in the serum of tuberculosis patients, providing an important theoretical basis for early diagnosis and differential diagnosis of disease. Isobaric tags for relative and absolute quantitation and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) technology is concerned and widely used by researchers because of its strong separation ability, wide analysis range, high sensitivity, high specificity, and low detection limit. This article reviews the researches on serum proteomics of tuberculosis patients based on iTRAQ-2DLC-MS/MS technology.

Key words: Tuberculosis, Proteomics, Isotope labeling, Chromatography, liquid, Tandem mass spectrometry, Laboratory techniques and procedures, Review literature as topic

Xin HU,Wushouer Qimanguli·. Research progress of isobaric tags for relative and absolute quantitation and two-dimensional liquid chromatography-tandem mass spectrometry in the diagnosis of tuberculosis[J]. Journal of Tuberculosis and Lung Health , 2018, 7(3): 217-220. doi: 10.3969/j.issn.2095-3755.2018.03.015

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