Abstract: Objective To understand the tuberculosis incidence and it’s variation trend in Panyu district of Guangzhou in order to provide evidence for tuberculosis control strategies development.Methods Prospective cohort study was used for this survey. 86 212 residents in Donghuan street and Shilou toanship in Panyu were confirmed to be the cohort based on the baseline survey. One year cohort observation and terminal survey were conducted to provide data for the tuberculosis incidence analysis.Results (1) The prevalence of active pulmonary tuberculosis was 103.23/100 000 in which smear positive tuberculosis was 38.28/100 000. (2) The standardized incidence rate of active pulmonary tuberculosis was 27.53/100 000 and 16.83/100 000 of smear positive tuberculosis. (3) Among the 18 new cases, 14 were male and 4 were female, and 5 were in 35-44 years age group.Conclusion A large-scale cohort were established in Guangdong province and tuberculosis incidence was obtained, and the male accounted the most in new cases.