Abstract:

Objective Clinical symptoms of non-tuberculous mycobacterium (NTM) diseases are similar to Mycobacterium tuberculosis (MTB) diseases, while the medication regimens are different, and it is difficult for differential diagnosis between NTM diseases and tuberculosis. How to identify NTM and MTB quickly and effectively is of great significance for the correct diagnosis and treatment of diseases.Methods Collecting sputum specimens from 495 outpatient and inpatient participants. The specimens were analyzed by spoligotyping and rapid culture of BACTEC MGIT 960 Mycobacterium tuberculosis (BACTEC MGIT 960 culture method ), respectively, and compared the two methods. Evaluated the diagnostic value including the sensitivity, specificity and accuracy of spoligotyping using culture as golden standard.Results Of 495 cases with suspected tuberculosis sputum specimens, specimens of 426 cases (86.1%,426/495) were detected with Mycobacterium tuberculosis and 69 cases (13.9%,69/495) with non-tuberculosis mycobacteria by using culture, and 420 cases (84.8%,420/495) were Mycobacterium tuberculosis and 75 cases (15.2%,75/495) with non-tuberculosis mycobacteria by using spoligotyping. Compared with the MGIT 960 culture method, the sensitivity, specificity, positive predictive value, negative predictive value, diagnostic coincidence rate and Kappa value of the spacer oligonucleotide microarray classification technology were 96.7% (412/426), 88.4% (61/69), 98.1% (412/420), 81.3% (61/75), 95.6% (473/495) and 0.821, respectively.Conclusion Compared with the MGIT 960 culture method, spoligotyping can quickly and accurately identify Mycobacterium tuberculosis and non-tuberculosis mycobacterium with high sensitivity and specificity. This method can provide effective results for timely diagnosis and treatment of patients and has a certain value of clinical promotion.

Key words: Mycobacteria, atypical, Diagnosis, differential, Spoligonucleotide probes, Culture techniques, Data interpretation, statistical