Diagnostic value of ELISA in HIV-infected patients with tuberculosis

doi:10.3969/j.issn.2095-3755.2018.04.013

Abstract

Abstract:

Objective To investigate the clinical diagnostic value of enzyme-linked immunosorbent assay (ELISA) in HIV-infected patients with tuberculosis.Methods 95 HIV-infected patients with tuberculosis and 19 HIV-infected patients with non-tuberculosis lung disease were selected as subjects who were diagnosed in Hunan Chest Hospital from January 2015 to September 2018. The positive detection rates of tuberculosis were analyzed with GeneXpert MTB/RIF detection technology (“GeneXpert”), BACTEC MGIT 960 detection technology (“MGIT 960”) and ELISA in whole blood sample. Analyzing the sensitivity, specificity, positive and negative predictive value of the three detection techniques for diagnosing HIV-infected patients with tuberculosis based on clinical diagnosis.Results In 95 HIV-infected patients with tuberculosis, the positive rates of GeneXpert, MGIT 960 and ELISA were 42.11% (40/95), 47.37% (45/95), and 67.37% (64/95) respectively, the positive rate of ELISA was significantly higher than that of GeneXpert and MGIT 960, and the difference was statistically significant (χ ²=50.031,P=0.000). In 19 HIV-infected patients with no-tuberculosis lung disease, the number of positive detection patients were 0, 0 and 3 in three groups. Based on clinical diagnosis, the sensitivity of GeneXpert was 42.11% (40/95), the specificity was 100.00% (19/19), the positive predictive value was 100.00% (40/40), and the negative predictive value was 25.68% (19/74); the MGIT 960 assay was 47.37% (45/95), 100.00% (19/19), 100.00% (45/45), 27.54% (19/69); and the ELISA was 73.56% (64/87), 84.21% (16/19), 95.52% (64/67), and 41.03% (16/39) respectively.Conclusion The detection of ELISA method is rapid and convenient with a high sensitivity, and it is suitable for clinical diagnosis of HIV-infected patients with tuberculosis.

Key words: HIV infections, Tuberculosis, Comorbidity, Enzyme-linked immunosorbent assay, Laboratory techniques and procedures, Comparative study

Yan-yan YU,Yun-hong TAN,Zhen-hua CHEN,Zhong-nan CHEN,Bin-bin LIU,Jue WANG. Diagnostic value of ELISA in HIV-infected patients with tuberculosis[J]. Journal of Tuberculosis and Lung Health , 2018, 7(4): 288-292. doi: 10.3969/j.issn.2095-3755.2018.04.013

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检测方法	临床诊断(例)		敏感度(%) (95%CI)	特异度(%) (95%CI)	阳性预测值(%) (95%CI)	阴性预测值(%) (95%CI)
检测方法	HIV并发结核病	HIV并发非结核性肺疾病	敏感度(%) (95%CI)	特异度(%) (95%CI)	阳性预测值(%) (95%CI)	阴性预测值(%) (95%CI)
GeneXpert检测			42.11 (32.68~52.16)	100.00	100.00	25.68 (17.1~36.66)
阳性	40	0
阴性	55	19
MGIT 960培养			47.37 (37.63~57.32)	100.00	100.00	27.54 (18.4~39.05)
阳性	45	0
阴性	50	19
ELISA试验			73.56 (63.44~81.69)	84.21 (62.43~94.48)	95.52 (87.64~98.46)	41.03 (25.98~56.59)
阳性	64	3
阴性	23	16