Abstract: Objective To evaluate the diagnostic value of electronic bronchoscopy in diagnosis of culture ne-gative pulmonary tuberculosis with tracheobronchial tuberculosis(TBTB).Methods The data of 78 patients of culture negative pulmonary tuberculosis with TBTB who were hospitalized in our hospital from January 2010 to August 2012 were analyzed,including the pathological classification of electronic bronchoscope and intratracheal lesions distribution before treatment. The results of brush examination,bronchoalveolar lavage fluid(BALF)smear,BALF culture,pathological biopsy and 3 sputum smear examinations after bronchoscope were compared.Results Seventy-two out of 78 cases’ electronic bronchoscope had abnormal display. And the main pathological classification were inflammatory infiltration(type Ⅰ100.0%,78/78),granulation hyperplasia(type Ⅲ 37.2%,29/78)and scar stricture(type Ⅳ34.6%,27/78).The positive rates of single test by brush,BALF smear,BALF culture,sputum smear after bronchoscope and pathological biopsy were 33.3％(26/78),39.7％(31/78),12.8％(10/78),26.9％(21/78)and 66.7%(28/42)respectively,and the differences among these five methods had statistically significance(χ²=38.94,P<0.01).The positive rate of bacteriology joint detection was 62.8%(49/78),and in biopsy combined increased significantly to 78.2％(61/78).Conclusion Comparing to single methods,different methods of bronchoscopy combination can improve the diagnosis of culture negative pulmonary tuberculosis with TBTB.