Abstract:

Objective To explore amplification of cross primer amplification (CPA) technique in tuberculosis (TB) prevention and control.Methods Sputum specimens were collected from the TB suspects who visited TB clinic of Wuchang TB Dispensary for seeking health care during May 2017 to March 2018. Diagnosis to those TB suspects were performed following the WS 288-2008 Diagnostic Criteria for Tuberculosis. Finally 331 cases were confirmed as TB including 267 cases were pulmonary TB while 64 cases were extra-pulmonary TB. Sputum smear, sputum culture and CPA were performed for TB diagnosis and the effectiveness of CPA was evaluated.Results Among 331 confirmed TB cases, 66 cases were sputum smear positive while 265 cases were smear negative; 136 cases were sputum culture positive while 195 cases were culture negative; 137 cases were CPA positive while 194 cases were CPA negative. When culture was as the standard, the sensitivity and specificity of CPA were 98.94% (186/188) and 92.31% (132/143) respectively; when clinical diagnosis was as the standard, the sensitivity and specificity of sputum smear were 23.97% (64/267) and 96.88% (62/64), the sensitivity and specificity of sputum culture were 48.69% (130/267) and 90.63% (58/64), the sensitivity and specificity of CPA were 51.31% (137/267) and 96.88%(62/64).Conclusion CPA is a new thermostatically diagnostic technology for tuberculosis, which is simple, fast and stable. It has higher sensitivity and specificity on Mycobacterium tuberculosis detection, can significantly improve the positive detection rate of TB. It is suitable for application in tuberculosis laboratories at county/district level.

Key words: Tuberculosis, pulmonary, Crossing priming amplification, Evaluation research