Abstract: Objective To acquire the job burnout situation of the anti-tuberculosis (anti-TB) medical staff in the center for disease control and prevention (CDC) at basic level, and to further analyze the reasons and explore the solution for preventing and remitting job burnout among anti-TB medical staff preliminarily.Methods All 26 anti-TB medical staff who worked in county CDC in two cities of Yunnan province (including clinical doctors, project managers, radiologists, and laboratory staff) accepted the individual interviews referring to job burnout.Results 85% (2/26) anti-TB medical staff showed strong sense of job burnout, and 69% (18/26) expected to leave anti-TB positions. The main reasons were as following: high working pressure, high risk of occupational exposure, low income, the misunderstanding and prejudice resulted from lacking of awareness of TB and insufficient leadership attention.Conclusion Job burnout phenomena among basic level anti-TB medical staff are relatively common, and the degree of burnout is severe. We should improve the working environment and mental health from policy, system, organization, human resource configuration to prevent and reduce the occurrence of burnout.