Abstract: Objective To improve the management efficiency of preventive treatment for latent tuberculosis infection(LTBI), explore the new measures to increase the compliance of LTBI preventive treatmentMethods Management efficiency of DOT and whole process management for LTBI preventive therapy had been compared during 2010—2014. TB outbreaks occurred in Neusoft Institute of Information and Dalian Ocean University were set in DOT group. The group consisted of 292 cases of close contacts, in which 130 cases were with latent infection. Other TB outbreaks happened in Dalian Technology University, Dalian Jiaotong University were set in whole process management group (control group). The group consisted of 244 cases of close contacts, in which 91 cases were LTBI. The rate of compliance and regular treatment were observed, and the causes of interruption of treatment were investigated. SPSS 19.0 was used for statistical analysis. Chi-square text was used compared rates and P value less than 0.05 were considered statistically significant. Results The acceptance rate of preventive treatment for LTBI, regularly therapy rate in the DOT and full management was 90.0%(117/130),90.6%(106/117), and 67.0%(61/91), 42.6%(26/61) respectively, χ²=18.0, 48.2, P values less than 0.05. The total rate of adverse drug reactions was 4.5%(8/178). In which the rate of DOT and full management group was 4.3 %(5/117) and 4.9%(3/61) respectively. The reasons of treatment interruption in the preventive treatment for LTBI were investigated(total 46 cases: 11 cases were in DOT group, and the rest 35 cases were in management group). In the DOT group, the main reasons were drugs side reactions and subjective neglect, accounted 27.3%(3/11) and 27.3% (3/11) respectively. While in the full management group, the main causes of treatment interruption were the fear of adverse drug reactions and subjective neglect, accounted 34.3%(12/35) and 28.6%(10/35) respectively. One-year-follow-ups after TB outbreaks, secondary attack rates in TST<15 mm group, regularly treatment with TST≥15 mm group, and inregularly treatment with TST≥15 mm group, were 1.6%(5/315),0.0% and 2.2%(2/89) respectively. Conclusion DOT will play the irreplaceable role in the fields of improving the compliance of preventive treatment for LTBI and promoting regularly preventive therapy.