Abstract: Objective To acquire the status of latent tuberculosis infection (LTBI) among village doctors and compare the concordance between QuantiFERON-TB Gold In-Tube(QFT-GIT)and tuberculin skin test (TST, using purified protein derivative of tuberculin,PPD).Methods A cross-sectional study was adopted in Linhe district of Bayan Nur City of Inner Mongolia in November, 2011. The questionnaire was used to collect information. Both of QFT-GIT and TST were used to detect the LTBI among 227 village doctors. Two hundred and twenty-seven questionnaires were issued, and the overall response rate was 100.0%. Statistical indexes, such as proportion, rate were used to describe village doctors’ basic information and the rate of LTBI. The concordance of QFT-GIT and TST was evaluated with Kappa value.Results 227 village doctors completed QFT-GIT and excluded 2 indeterminate results, 99 of 225 village doctors’ QFT-GIT results were positive, the rate of LTBI was 44.0% (99/225). Two hundred and twenty-six village doctors completed TST, the rates of LTBI were 59.3% (134/226) with cutoff of 5 mm, 41.2% (93/226) with cutoff of 10 mm and 22.6% (51/226) with cutoff of 15 mm respectively. The Kappa values were 0.415 with cutoff of 5 mm, 0.444 with cutoff of 10 mm and 0.358 with cutoff of 15 mm respectively.Conclusion The rate of LTBI among 227 village doctors in Linhe district was higher,the agreement of these two methods was general. We should conduct tuberculosis infection control measures at village clinic, so that reduce the LTBI of village doctors.