Abstract: Objective To investigate the streptomycin resistance of anti-TB drugs in patients with pulmonary tuberculosis (PTB) in Suzhou city. Methods Retrospectively analysis were carried out on the 1749 patients with PTB of Suzhou city between September 2008 and May 2011 to investigate the streptomycin-resistance rate and the incidence of resistance rate of streptomycin combination with isoniazid, rifampicin or ethambutol and the streptomycin resistance rate’s composition in the multidrug-resistant TB(MDR-TB). The Chi-square test was used for statistical analysis, P<0.05 is considered statistically significant. Results In recent 3 years in Suzhou city, the total streptomycin-resistance rate was 24.7%(432/1749) and 87.4%(160/183) of MDR-TB cases were streptomycin-resistant. The streptomycin-resistance rate in retreatment cases was 44.3%(160/361), which is significantly higher than 19.6%(272/1388) in new cases (χ²=94.16,P<0.01). 93.5％(87/93)of patients in retreatment cases were streptomycin-resistant, which is also significantly higher than 81.1％(73/90)in new cases (χ²=6.44,P<0.05). Conclusion The streptomycin-resistance rate had highest single drug resistance in patients with PTB in Suzhou city, and streptomycin-resistance rate were high both in new and retreatment of MDR-TB patients.