Abstract: Objective To estimate the required funds for diagnosis and treatment of pulmonary tuberculosis (PTB) patients and to provide reference for implementing National Tuberculosis Control Program(NTP) and improving financing mechanism.Methods Based on the estimation unit of one prefecture, the annual diagnosis and treatment fund demand per capita and per case of common TB patient and MDR-TB patient were calculated respectively, the fund demand nationwide from 2011—2015 were estimated. In accordance with the sixth national census, central government transfer payment program for TB control and the extension plan of programmatic management of MDR-TB from 2011—2015, 334 prefectures nationwide implementing TB control program and basic parameters for one prefecture (3.895 million population, 8 counties and 127 towns/communities) were determined. In terms of the final evaluation report of NTP (2001—2010) and the baseline survey result of drug resistance from 2007—2008 nationwide, the number of patients diagnosed and reported annually in one prefecture was estimated.Results 17630000 yuan was required for patient diagnosis and treatment in one prefecture on average in one year, with 4.53 yuan per capita, in which common TB patients required 13000000 yuan (74%), with 3.34 yuan per capita, while MDR-TB required 4630000 yuan (26%), with 1.19 yuan per capita. On average, the required fund of one common TB patient for diagnosis, treatment and patient care was 4151 yuan, while one MDR-TB patient required 42129 yuan. In accordance with the NTP (2011—2015) and MDR-TB management tentative expansion plan, a total of 5.02 billion yuan, 5.12 billion yuan and 5.27 billion yuan were required during 2013 to 2015 nationwide respectively.Conclusion The method and result of estimation will act as a scientific base for estimating financing of TB diagnosis and treatment, and also provide reference for estimating local required funds and improving financing mechanism.