Research progress on free DNA detection of Mycobacterium tuberculosis of non-sputum samples in tuberculosis diagnosis

doi:10.3969/j.issn.2096-8493.20210069

Abstract

Abstract:

Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis, pulmonary tuberculosis is the most common. Laboratory test mainly respiratory specimens (sputum, lavage) as test objects, however, the test effective is unfavorable for children, extrapulmonary tuberculosis, and TB/HIV or AIDS patients. Thus, new diagnosis method based on non-sputum samples in continuous research in order to positive test rate of above population. Free DNA (cfDNA) is a DNA fragment released from primitive cells into various body fluids, which has a broad application prospect in the diagnosis of various diseases. In the article, application, factors affecting extraction pretreatment, extraction and detection methods of Mycobacterium tuberculosis cfDNA detection in blood or urine specimen will be discussed by authors, in combination with literature and clinical experience.

Key words: Mycobacterium tuberculosis, DNA, Etiological diagnosis, Brine, Blood, Review literature as topic

LI Jing, ZHANG Yan, WU Qian-hong. Research progress on free DNA detection of Mycobacterium tuberculosis of non-sputum samples in tuberculosis diagnosis[J]. Journal of Tuberculosis and Lung Disease , 2021, 2(3): 277-282. doi: 10.3969/j.issn.2096-8493.20210069

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