Abstract: Objective To understand the financing requirement, implementation and gap for the tuberculosis(TB) prevention and control at provincial, prefecture and county levels in Anhui province in 2011.Methods TB prevention and control institutes at provincial, prefecture and county levels made their own work plans and calcula-ted relevant funds requirements of 2011 according to the local economic situations, based on the scheme of TB control basic work plan and funds requirement. The forms of basic work plan and funds requirement for TB prevention and control were filled out and submitted to higher administrative levels. The forms and data reported from different prefectures were gathered and summarized by provincial TB prevention and control institute. The funds budget software provided by China CDC based on Microsoft Access database was used to calculate the funds requirement for TB prevention and control.Results The total funds required for TB prevention and control of Anhui province in 2011 was 93.26 million Yuan; however, the special funds provided by central finance, local governments at all levels and global funds were 21.64, 34.67 and 9.93 million Yuan respectively, the financing gap was 27.02 million Yuan, which accounted for 29.0% of the required financial support.Conclusion The calculation shows that funding gap has existed in all types of TB prevention and control activities at all levels in Anhui.