Abstract: Objective To understand the knowledge, behavior and attitude of the freshmen on tuberculosis (TB) prevention and control in a college of Dalian, and to provide scientific basis for health education on TB prevention and control in college students in Dalian. Methods Questionnaire survey was conducted in 4194 freshmen in October 2014. The questionnaire was designed according to the Core Information of TB Prevention and Control (2010 edition), which included the basic situation of students, 7 core information of TB prevention and control, attitude, behavior and the ways to gain TB knowledge. A total of 3819 questionnaires were distributed and 3662 effective questionnaires were taken back. SPSS 17.0 was used to analyze, chi-square test was applied for comparison between groups. P<0.05 was statistically significant. Results The overall awareness rate of TB prevention and control was 77.1% (19 757/25 634). The awareness rate of male 75.7% (8474/11 198) was significantly less than female 78.2% (11 283/14 436) (χ²=22.030, P<0.05). The awareness rate of students with city residence was 77.2% (11 701/15 154), which was higher than the rural residence (76.9%, 8056/10 480), and there was no statistical significance (χ²＝0.414, P>0.05). The awareness rate of students with parents graduated from high school or above was 77.2% (10 603/13 727), which was higher than that with high school below, and the difference was not statistically significant (χ²=0.475, P>0.05). For the ways to get TB prevention and control knowledge, from the campus health promotion activities was 52.9% (1939/3662). The awareness rates of “Keeping ventilated every day”, “Introduce the TB related knowledge to family members and friends actively”, “Eat regular meals, to guarantee the nutrition” were higher, which were 95.7% (3506/3662), 78.1% (2861/3662) and 86.5% (3166/3662) respectively. Conclusion TB awareness of college freshmen is generally low. By the cooperation of TB control institutes and schools, we should promote TB health promotion, and that is a main way to improve the awareness on TB prevention and control.