Abstract: Objective To analyze the characteristics of TB epidemic situation in colleges in Dalian, provide the basis for the future TB control. Methods From 2010 to 2014, Dalian city carried out freshmen TB health check, the screening of TB close contacts and the preventive treatment for LTBI, and so on. The trends of TB pre-valence in freshmen and TB incidence in colleges were observed by trend Chi-square test, and P<0.05 was consi-dered statistically significant. Results The TB prevalence of freshmen in colleges between 2010 and 2014 were 73.4/10⁵ (34/46 290), 53.9/10⁵ (27/50 050), 66.0/10⁵ (35/53 030), 96.3/10⁵ (52/53 986) and 75.4/10⁵ (41/54 383) (χ²_trend＝0.028, P>0.05) respectively. The proportion of TST≥15 mm were 5.2% (2428/46 290), 7.8% (3925/50 050), 7.5% (3953/53 030), 6.0% (3243/53 986) and 5.8% (3134/54 383) among freshmen (χ²_trend＝1.301, P>0.05). The TB incidence between 2010 and 2014 were 54.9/10⁵ (154/280 399), 55.1/10⁵ (161/291 974), 43.1/10⁵ (130/301 307), 41.9/10⁵ (132/314 708) and 44.7/10⁵ (146/326 326) (χ²_trend＝2.648, P>0.05). The smear positive TB incidence were 7.1/10⁵ (20/280 399), 9.9/10⁵ (29/291 974), 3.7/10⁵ (11/301 307), 5.4/10⁵ (17/314 708) and 5.2/10⁵ (17/326 326) respectively (χ²_trend＝1.232, P>0.05). In the past 4 years, there were 80 TB cases detected by the screening of close contacts, and the detection rate was 228.3/10⁵ (80/35 042), which was significantly higher than 73.3/10⁵ (189/257 739) among freshmen (χ²＝77.537, P<0.05). The incidence of sporadic TB was observed by removing secondary cases in clusters. Their incidence were 49.9/10⁵ (140/280 399), 47.3/10⁵ (138/291 974), 41.8/10⁵ (126/301 307), 35.6/10⁵ (112/314 708) and 34.6/10⁵ (113/326 326). It showed a significant decreasing trend (χ²_trend＝3.838, P<0.05). The incidence of secondary clustered cases were 5.0/10⁵ (14/280 399), 7.9/10⁵ (23/291 974), 1.3/10⁵ (4/301 307), 6.4/10⁵ (20/314 708) and 10.1/10⁵ (33/326 326) (χ²_trend＝0.695, P>0.05), whose characteristic of changes, from 2010 to 2014, was similar to the TB incidence. Conclusion School TB prevention and control work made some progress in Dalian city, but early finding of clustered cases still faces serious challenges which requires innovative strategies.